What is the catalog number for Goat Interleukin 8 (IL8) ELISA Kit?

The catalog number for Goat Interleukin 8 (IL8) ELISA Kit is orb1736523.

What sizes are available for Goat Interleukin 8 (IL8) ELISA Kit?

Goat Interleukin 8 (IL8) ELISA Kit is available in the following size(s): 48 Tests, 96 Tests.

How many pack sizes does Goat Interleukin 8 (IL8) ELISA Kit come in?

Goat Interleukin 8 (IL8) ELISA Kit is available in 2 different pack sizes.

Is Goat Interleukin 8 (IL8) ELISA Kit in stock?

Goat Interleukin 8 (IL8) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Goat Interleukin 8 (IL8) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

IL-8; CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

UniProt

P36925

Reactivity

Goat

Field of Research

Immunology & Inflammation; Cell Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

6.2 pg/mL

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IL8 in the samples is then determined by comparing the OD of the samples to the standard curve