What is the catalog number for LEF1 Antibody?

The catalog number for LEF1 Antibody is orb19285.

What sizes are available for LEF1 Antibody?

LEF1 Antibody is available in the following size(s): 100 µg.

Is LEF1 Antibody in stock?

LEF1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

LEF1 Antibody

Goat polyclonal antibody to LEF1

Product Specifications

Product Name Alternative

Anti LEF1 antibody, anti lymphoid enhancer-binding factor 1 antibody, anti HGNC:6551 antibody, anti DKFZp586H0919 antibody, anti TCF1ALPHA antibody, anti lymphoid enhancer binding factor-1 antibody

Reactivity

Human

Target

LEF1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QHEQRKEQEPKRPH

Field of Research

Epigenetics, Neuroscience, Signaling Pathways, Stem Cells, Wnt

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml

Molecular Weight

44.2; 41.2; 42.7; 34.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3;21 (11) :2703-14.; PMID: 12032083) . Recommended concentration: 1-3 μg/ml.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, FC, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.