ROBO1/DUTT1 (Internal) Antibody
Goat polyclonal antibody to ROBO1
Product Specifications
Product Name Alternative
Anti ROBO1 antibody, anti DUTT1 antibody, anti roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody, anti HGNC:10249 antibody, anti FLJ21882 antibody, anti SAX3 antibody, anti axon guidance receptor antibody, anti roundabout 1 antibody, anti MGC131599 antibody, anti MGC133277 antibody
Reactivity
Human
Target
ROBO1 / DUTT1 (Internal)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
GDVDLSNKINEMK
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 1 μg/ml, IHC-P: 2 μg/ml
Molecular Weight
181; 177
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1) . Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3 μg/ml.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IF, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6091
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