VPS29 (Internal) Antibody
Goat polyclonal antibody to VPS29
Product Specifications
Product Name Alternative
Anti VPS29 antibody, anti vacuolar protein sorting 29 (yeast) antibody, anti DC15 antibody, anti DC7 antibody, anti DKFZp564F0223 antibody, anti FLJ20492 antibody, anti PEP11 antibody, anti retromer protein antibody, anti vacuolar protein sorting 29 antibody, anti vacuolar protein sorting 29 (yeast homolog) antibody, anti vacuolar sorting protein VPS29/PEP11 antibody, anti x 007 protein antibody
Reactivity
Human
Target
VPS29 (Internal)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CTGNLCTKESYDYLK
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
20.5; 20.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
51699
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