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VPS29 (Internal) Antibody

Goat polyclonal antibody to VPS29

Product Specifications

Product Name Alternative

Anti VPS29 antibody, anti vacuolar protein sorting 29 (yeast) antibody, anti DC15 antibody, anti DC7 antibody, anti DKFZp564F0223 antibody, anti FLJ20492 antibody, anti PEP11 antibody, anti retromer protein antibody, anti vacuolar protein sorting 29 antibody, anti vacuolar protein sorting 29 (yeast homolog) antibody, anti vacuolar sorting protein VPS29/PEP11 antibody, anti x 007 protein antibody

Reactivity

Human

Target

VPS29 (Internal)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CTGNLCTKESYDYLK

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

20.5; 20.9

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

51699

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