W-7 (hydrochloride)

W-7 hydrochloride is a selective calmodulin antagonist. W-7 hydrochloride inhibits the Ca2+-calmodulin-dependent phosphodiesterase and myosin light chain kinase with IC50 values of 28 μM and 51 μM, respectively. W-7 hydrochloride induces apoptosis and has antitumor and vascular relaxing activity. W-7 hydrochloride is a blocker of Kv4.3 and can be used for research of arrhythmias[1][2][3][4][5][6].

Product Specifications

CAS Number

[61714-27-0]

UNSPSC

12352005

Hazard Statement

H315, H319, H335

Target

Apoptosis; Calmodulin; Myosin; Phosphodiesterase (PDE) ; Potassium Channel

Type

Reference compound

Related Pathways

Apoptosis; Cytoskeleton; Membrane Transporter/Ion Channel; Metabolic Enzyme/Protease

Applications

Cancer-Kinase/protease

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/w-7-hydrochloride.html

Concentration

10mM

Purity

99.96

Solubility

DMSO : 250 mg/mL (ultrasonic) |H2O : 1.43 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=S(C1=C2C=CC=C(Cl)C2=CC=C1)(NCCCCCCN)=O.[H]Cl

Molecular Formula

C16H22Cl2N2O2S

Molecular Weight

377.33

Precautions

H315, H319, H335

References & Citations

[1]H Hidaka, et al. N- (6-aminohexyl) -5-chloro-1-naphthalenesulfonamide, a Calmodulin Antagonist, Inhibits Cell Proliferation. Proc Natl Acad Sci U S A. 1981 Jul;78 (7) :4354-7.|[2]M Asano. Divergent Pharmacological Effects of Three Calmodulin Antagonists, N- (6-aminohexyl) -5-chloro-1-naphthalenesulfonamide (W-7), Chlorpromazine and Calmidazolium, on Isometric Tension Development and Myosin Light Chain Phosphorylation in Intact Bovine Tracheal Smooth Muscle. J Pharmacol Exp Ther. 1989 Nov;251 (2) :764-73.|[3]H Itoh, et al. Direct Interaction of Calmodulin Antagonists With Ca2+/calmodulin-dependent Cyclic Nucleotide Phosphodiesterase. J Biochem. 1984 Dec;96 (6) :1721-6.|[4]Shigeyuki Yokokura, et al. Calmodulin Antagonists Induce Cell Cycle Arrest and Apoptosis in Vitro and Inhibit Tumor Growth in Vivo in Human Multiple Myeloma. BMC Cancer. 2014 Nov 26;14:882.|[5]Qu YJ, et al. W-7 modulates Kv4.3: pore block and Ca2+-calmodulin inhibition. Am J Physiol Heart Circ Physiol. 2007 May;292 (5) :H2364-77.|[6]Gbadebo TD, et al. Calmodulin inhibitor W-7 unmasks a novel electrocardiographic parameter that predicts initiation of torsade de pointes. Circulation. 2002 Feb 12;105 (6) :770-4.