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Prolyl Endopeptidase/PREP Antibody

Goat polyclonal antibody to PREP

Product Specifications

Product Name Alternative

Anti PREP antibody, anti prolyl endopeptidase antibody, anti HGNC:9358 antibody, anti MGC16060 antibody, anti PE antibody, anti PEP antibody, anti OTTHUMP00000040498 antibody, anti dJ355L5.1 (prolyl endopeptidase) antibody, anti post-proline cleaving enzyme antibody

Reactivity

Human

Target

Prolyl Endopeptidase / PREP

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QYIVGRSRKQSNP

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

80.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5550

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