Prolyl Endopeptidase/PREP Antibody
Goat polyclonal antibody to PREP
Product Specifications
Product Name Alternative
Anti PREP antibody, anti prolyl endopeptidase antibody, anti HGNC:9358 antibody, anti MGC16060 antibody, anti PE antibody, anti PEP antibody, anti OTTHUMP00000040498 antibody, anti dJ355L5.1 (prolyl endopeptidase) antibody, anti post-proline cleaving enzyme antibody
Reactivity
Human
Target
Prolyl Endopeptidase / PREP
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QYIVGRSRKQSNP
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
80.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5550
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