MDM2 (isoform) Antibody
Goat polyclonal antibody to MDM2
Product Specifications
Product Name Alternative
MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), HGNC:6973, MGC71221, hdm2, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein, p53-binding protein MDM2, ubiquitin-protein ligase E3 Md
Reactivity
Human
Target
MDM2 (isoform)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DELSGERQRKRHKSD
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.3-1.0 μg/ml
Molecular Weight
56
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 55kDa band observed in Human Liver lysates (calculated MW of 56.0kDa according to NP_002383.2) . In transfected HEK293 transiently expressing MDM2 bands of approx. 48kDa, 60KDa, 70kDa and 90kDa are observed. These bands are not observed in the non-transfected HEK293. The 80kDa band is endogenously epressed in the negative control. Recommended concentration: 0.3-1.0 μg/ml.
Prediction Reactivity
Bovine, Canine, Feline, Porcine, Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4193
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