TRAF1 Antibody
Goat polyclonal antibody to TRAF1
Product Specifications
Product Name Alternative
Anti TRAF1 antibody, anti TNF receptor-associated factor 1 antibody, anti EBI6 antibody, anti MGC:10353 antibody
Reactivity
Human
Target
TRAF1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KLQSPKHAYVKDD
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.3-1.0 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
46.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50-55kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2kDa according to NP_005649.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhuber et al , Am J Respir Cell Mol Biol. 2000 Feb;22 (2) :150-6. PMID: 10657935) . Recommended concentration: 0.3-1.0 μg/ml.
Prediction Reactivity
Mouse
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
7185
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