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TRAF1 Antibody

Goat polyclonal antibody to TRAF1

Product Specifications

Product Name Alternative

Anti TRAF1 antibody, anti TNF receptor-associated factor 1 antibody, anti EBI6 antibody, anti MGC:10353 antibody

Reactivity

Human

Target

TRAF1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KLQSPKHAYVKDD

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3-1.0 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

46.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50-55kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2kDa according to NP_005649.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhuber et al , Am J Respir Cell Mol Biol. 2000 Feb;22 (2) :150-6. PMID: 10657935) . Recommended concentration: 0.3-1.0 μg/ml.

Prediction Reactivity

Mouse

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

7185

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