Engrailed-2 Antibody

Goat polyclonal antibody to EN2

Product Specifications

Product Name Alternative

Anti Engrailed-2 antibody, anti engrailed homolog 2 antibody, anti EN2 antibody, anti AUTS1 antibody

Reactivity

Human, Mouse

Target

Engrailed-2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NHSTTAKEGKSDSE

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

34.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 30kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2kDa according to Human NP_001418.2 and of 33.8kDa according to Mouse NP_034264.1 and of 33.7kDa according to Rat NP_001102684.1) . Recommended concentration: 0.1-0.3 μg/ml.

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.