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DEAD-box protein 6 Antibody

Goat polyclonal antibody to DDX6

Product Specifications

Product Name Alternative

Anti DEAD-box protien 6 antibody, anti DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 antibody, anti DDX6 antibody, anti HLR2 antibody, anti P54 antibody, anti RCK antibody, anti DEAD box-6 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD) antibody

Reactivity

Human

Target

DEAD-box protein 6

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

AEYHSEPVEDEKP

Field of Research

Cancer, Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

53.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 53.2kDa according to NP_NP_004388.1) . In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 53kDa protein is observed in both. Recommended concentration: 0.1-0.3 μg/ml.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1656

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