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PCK1/PEPCKC (internal) Antibody

Goat polyclonal antibody to PCK1

Product Specifications

Product Name Alternative

Anti PCK1 antibody, anti phosphoenolpyruvate carboxykinase 1 (soluble) antibody, anti HGNC:8724 antibody, anti MGC22652 antibody, anti PEPCK1 antibody, anti PEPCKC antibody, anti PEP carboxykinase antibody, anti cytosolic phosphoenolpyruvate carboxykinase 1 antibody, anti phosphoenolpyruvate carboxylase antibody, anti phosphopyruvate carboxylase antibody, anti PEPCK-C antibody

Reactivity

Human

Target

PCK1 / PEPCKC (internal)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HVNWFRKDKEGK

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.3 μg/ml, IHC-P: 2 μg/ml

Molecular Weight

69.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in pyramidal cells. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5105

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