Recombinant Human Blood group Rh (D) polypeptide (RHD), partial, Biotinylated
Product Specifications
Product Name Alternative
(RHXIII) (Rh polypeptide 2) (RhPII) (Rhesus D antigen) (CD antigen CD240D)
Abbreviation
Recombinant Human RHD protein, partial, Biotinylated
Gene Name
RHD
UniProt
Q02161
Expression Region
388-417aa
Organism
Homo sapiens (Human)
Target Sequence
LNLKIWKAPHEAKYFDDQVFWKFPHLAVGF
Tag
N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Cardiovascular
Relevance
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
51.4 kDa
References & Citations
"Rh (D) antigen expression and isolation of a new Rh (D) cDNA isoform in human erythroleukemic K562 cells." Suyama K., Lunn R., Haller S., Goldstein J. Blood 84:1975-1981 (1994)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
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