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EMI2 Antibody

Goat polyclonal antibody to FBXO43

Product Specifications

Product Name Alternative

Anti FBXO43 antibody, anti EMI2 antibody, anti F-box protein 43 antibody, anti Fbx43 antibody, anti early mitotic inhibitor 2 antibody, anti ERP1 antibody

Reactivity

Mouse

Target

EMI2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

PGSAQSKRNLKRL

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3-1 μg/ml

Molecular Weight

78.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: This product has been successfully used on Rat Pachytene Spermatocytes showing a 71kDa band (Perrard et al, PLoS One. 2009 Oct 5;4 (10) :e7237. PMID: 19802389) . Recommended concentration: 1-3 μg/ml.Experiment Notes: This product has been successfully used in Immunofluorescence and Flow Cytometry on Rat meiotic Testis cells: (Perrard et al, PLoS One. 2009 Oct 5;4 (10) :e7237. PMID: 19802389) . Recommended concentration: 5-10ug/ml.

Prediction Reactivity

Bovine, Canine, Human, Porcine, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

286151

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