What is the catalog number for TRIM5/RNF88 (alpha isoform) Antibody?

The catalog number for TRIM5/RNF88 (alpha isoform) Antibody is orb19133.

What sizes are available for TRIM5/RNF88 (alpha isoform) Antibody?

TRIM5/RNF88 (alpha isoform) Antibody is available in the following size(s): 100 µg.

Is TRIM5/RNF88 (alpha isoform) Antibody in stock?

TRIM5/RNF88 (alpha isoform) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

TRIM5/RNF88 (alpha isoform) Antibody

Goat polyclonal antibody to TRIM5

Product Specifications

Product Name Alternative

Anti TRIM5 antibody, anti tripartite motif-containing 5 antibody, anti HGNC:16276 antibody, anti RNF88 antibody, anti tripartite motif protein 5 antibody, anti tripartite motif protein TRIM5 antibody, anti TRIM5alpha antibody, anti OTTHUMP00000069812 antibody, anti tripartite motif protein TRIM antibody, anti tripartite motif-containing protein 5 alpha antibody

Target

TRIM5 / RNF88 (alpha isoform)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CNIEKNENYQPK

Field of Research

Infectious Disease & Virology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 3-6μg/ml

Molecular Weight

56.3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6ug/ml.WB: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_149023.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.