Klotho Antibody
Goat polyclonal antibody to Klotho
Product Specifications
Product Name Alternative
Anti KL antibody, anti KLOT_HUMAN antibody, anti Klotho peptide antibody
Reactivity
Human
Target
Klotho
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KRDDAKYMYYLKK
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 4-6 μg/ml
Molecular Weight
116; 62.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Breast shows cytoplasm staining in luminla epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration, 4-6ug/ml.WB: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9365
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