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ZNF703 Antibody

Goat polyclonal antibody to ZNF703

Product Specifications

Product Name Alternative

Anti ZNF703 antibody, anti zinc finger protein 703 antibody, anti FLJ14299 antibody, anti ZNF503L antibody, anti hypothetical protein LOC80139 antibody

Reactivity

Human

Target

ZNF703

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ELDKKDQEPKPSPE

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml

Molecular Weight

58.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 28-30kDa band in human breast lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.2kDa according to NP_079345.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported An additional band of approx 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Prediction Reactivity

Canine, Porcine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

80139

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