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NOTCH3 Antibody

Goat polyclonal antibody to NOTCH3

Product Specifications

Product Name Alternative

Anti NOTCH3 antibody, anti Notch homolog 3 (Drosophila) antibody, anti HGNC:7883 antibody, anti CADASIL antibody, anti CASIL antibody, anti Notch (Drosophila) homolog 3 antibody, anti Notch homolog 3 antibody

Reactivity

Human

Target

NOTCH3

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QLGPQPEVTPKRQ

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, IHC-P: 5 μg/ml

Molecular Weight

244

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, FC, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

4854

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