VPS41 Antibody
Goat polyclonal antibody to VPS41
Product Specifications
Product Name Alternative
Anti VPS41 antibody, anti vacuolar protein sorting 41 (yeast) antibody, anti HGNC:12713 antibody, anti HVSP41 antibody, anti vacuolar assembly protein 41 antibody, anti vacuolar protein sorting 41 (yeast homolog) antibody, anti vacuolar protein sorting 41 homolog (S. cerevisiae) antibody, anti HVPS41 antibody, anti hVps41p antibody, anti vacuolar protein sorting 41 antibody
Reactivity
Human
Target
VPS41
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DHLKKDSQNKTLLK
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 1 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
98.5; 95.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
27072
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