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CFC1/Cripto-1 Antibody

Goat polyclonal antibody to CFC1

Product Specifications

Product Name Alternative

Anti CFC1 antibody, anti HTX2 antibody, anti CRYPTIC antibody, anti cripto, FRL-1, cryptic family 1 antibody, anti cripto-1 antibody, anti cryptic antibody, anti cryptic gene antibody, anti MGC133213 antibody, anti CR-1 antibody, anti FLJ77897 antibody

Reactivity

Mouse

Target

CFC1 / Cripto-1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HFTGRYCEHDQRRSEC

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 1-2 μg/ml

Molecular Weight

24.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testes lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

55997

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