CFC1/Cripto-1 Antibody
Goat polyclonal antibody to CFC1
Product Specifications
Product Name Alternative
Anti CFC1 antibody, anti HTX2 antibody, anti CRYPTIC antibody, anti cripto, FRL-1, cryptic family 1 antibody, anti cripto-1 antibody, anti cryptic antibody, anti cryptic gene antibody, anti MGC133213 antibody, anti CR-1 antibody, anti FLJ77897 antibody
Reactivity
Mouse
Target
CFC1 / Cripto-1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
HFTGRYCEHDQRRSEC
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1 μg/ml, IHC-P: 1-2 μg/ml
Molecular Weight
24.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testes lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
55997
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