β-Catenin (phospho-Ser675) rabbit pAb

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016],

Product Specifications

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs) . AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Product Name Alternative

CTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-catenin

UniProt

P35222

Swiss Prot

P35222

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized phosho peptide around human β-Catenin (Ser675)

Target

Catenin-β

Clonality

Polyclonal

Source

Rabbit

Applications

Concentration

1 mg/ml

Dilution

WB 1:1000-2000

Buffer

-20°C/1 year

Molecular Weight

92kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

92kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell junction . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse . Cytoplasm, cytoskeleton, cilium basal body . Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity) . Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation) . Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of cent