HMGA1 Antibody

Goat polyclonal antibody to HMGA1

Product Specifications

Product Name Alternative

Anti high mobility group AT-hook 1 antibody, anti High mobility group AT-hook protein 1 antibody, anti High mobility group protein A1 antibody, anti High mobility group protein-R antibody

Reactivity

Human

Target

HMGA1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

LASKQEKDGTEK

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 4 μg/ml

Molecular Weight

11.7; 10.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong nuclear staining. Recommended concentration, 4-6ug/ml.WB: Preliminary experiments gave an approx 50kDa band in Human Testis and Ovary lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 11.7kDa according to isoform a (also called HMG-I; NP_665906.1; NP_665908.1; NP_665911.1) and 10.7kDa according to isoform b ((also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1) . The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.