Histamine Receptor H2 Antibody
Goat polyclonal antibody to HRH2
Product Specifications
Product Name Alternative
Anti HRH2 antibody, anti histamine receptor H2 antibody, anti HGNC:5183 antibody, anti H2R antibody, anti gastric receptor 1 antibody, anti OTTHUMP00000161242 antibody
Reactivity
Human
Target
Histamine Receptor H2
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QEEKPLKLQVWSGTE
Field of Research
Pharmacology & Drug Discovery
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to Human NP_001354640.1) . Recommended concentration: 1-3μg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Molecular Weight
44.5; 46.9
References & Citations
Zhuang, Qian-Xing et al. Histamine Excites Striatal Dopamine D1 and D2 Receptor-Expressing Neurons via Postsynaptic H1 and H2 Receptors Mol Neurobiol, 55, 8059-8070 (2018)
Pubmed id
29498008
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
3274
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