What is the catalog number for NANOG Antibody?

The catalog number for NANOG Antibody is orb19063.

What sizes are available for NANOG Antibody?

NANOG Antibody is available in the following size(s): 100 µg.

Is NANOG Antibody in stock?

NANOG Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

NANOG Antibody

Goat polyclonal antibody to NANOG

Product Specifications

Product Name Alternative

Anti NANOG antibody, anti Nanog homeobox antibody, anti HGNC:20857 antibody, anti homeobox transcription factor Nanog antibody, anti homeobox transcription factor Nanog-delta 48 antibody

Reactivity

Human

Target

NANOG

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QNQRMKSKRWQKNN

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.03-0.1 μg/ml

Molecular Weight

34.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1) . Recommended concentration: 0.03-0.1 μg/ml.Experiment Notes: Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green) . Immunofluorescence: see also publication Raya A. et al., Nature. 2009 Jul 2;460 (7251) :53-9. Epub 2009 May 31. (PMID: 19483674) . This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.

Prediction Reactivity

Canine, Porcine

Tested Applications

ELISA, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.