NANOG Antibody
Goat polyclonal antibody to NANOG
Product Specifications
Product Name Alternative
Anti NANOG antibody, anti Nanog homeobox antibody, anti HGNC:20857 antibody, anti homeobox transcription factor Nanog antibody, anti homeobox transcription factor Nanog-delta 48 antibody
Reactivity
Human
Target
NANOG
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QNQRMKSKRWQKNN
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.03-0.1 μg/ml
Molecular Weight
34.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1) . Recommended concentration: 0.03-0.1 μg/ml.Experiment Notes: Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green) . Immunofluorescence: see also publication Raya A. et al., Nature. 2009 Jul 2;460 (7251) :53-9. Epub 2009 May 31. (PMID: 19483674) . This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Prediction Reactivity
Canine, Porcine
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
79923
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