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Osteopontin/SPP1 Antibody

Goat polyclonal antibody to Osteopontin

Product Specifications

Product Name Alternative

Anti-BNSP antibody, anti-Bone sialoprotein 1 antibody, anti-BSP I antibody, anti-BSPI antibody, anti-ETA1 antibody, anti-Nephropontin antibody, anti-OPN antibody, anti-Osteopontin antibody, anti-Secreted phosphoprotein 1 antibody, anti-SPP 1 antibody, anti-SPP-1 antibody, anti-SPP1 antibody, anti-Uropontin antibody

Reactivity

Human

Target

Osteopontin / SPP1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DPKSKEEDKHLK

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.1-0.3 μg/ml

Molecular Weight

35.4; 33.8; 32.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1) . Recommended concentration: 1-3 μg/ml.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

6696

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