Osteopontin/SPP1 Antibody
Goat polyclonal antibody to Osteopontin
Product Specifications
Product Name Alternative
Anti-BNSP antibody, anti-Bone sialoprotein 1 antibody, anti-BSP I antibody, anti-BSPI antibody, anti-ETA1 antibody, anti-Nephropontin antibody, anti-OPN antibody, anti-Osteopontin antibody, anti-Secreted phosphoprotein 1 antibody, anti-SPP 1 antibody, anti-SPP-1 antibody, anti-SPP1 antibody, anti-Uropontin antibody
Reactivity
Human
Target
Osteopontin / SPP1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DPKSKEEDKHLK
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.1-0.3 μg/ml
Molecular Weight
35.4; 33.8; 32.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 30kDa band observed in lysates of cell lines Jurkat and MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1) . Recommended concentration: 1-3 μg/ml.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6696
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