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Goat anti-CLCA1 Antibody

Goat polyclonal antibody to CLCA1

Product Specifications

Product Name Alternative

Anti CLCA1 antibody, anti chloride channel, calcium activated, family member 1 antibody, anti HGNC:2015 antibody, anti CaCC antibody, anti calcium activated chloride channel 1 antibody

Reactivity

Human

Target

CLCA1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

TVTSKTNKDTSK

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml

Molecular Weight

100

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 26kDa band in human duodenum, kidney and lung lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 100kDa according to NP_001276.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1179

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