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Pancreatic Polypeptide/PPY Antibody

Goat polyclonal antibody to PPY

Product Specifications

Product Name Alternative

Anti PPY antibody, anti pancreatic polypeptide antibody, anti HGNC:9327 antibody, anti PNP antibody, anti pancreatic polypeptide Y antibody

Reactivity

Human, Rat

Target

Pancreatic Polypeptide / PPY

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

TRPRYGKRHKEDT

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, IHC-P: 5 μg/ml

Molecular Weight

10.5; 11.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration, 3ug/ml.WB: Preliminary experiments gave an approx 35-38kDa band in human pancreas lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.

Prediction Reactivity

Canine, Mouse

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5539

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