RICTOR Antibody
Goat polyclonal antibody to RICTOR
Product Specifications
Product Name Alternative
Anti RICTOR antibody, anti Rictor antibody, anti KIAA1999 antibody, anti AVO3 antibody, anti MGC39830 antibody, anti likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae) antibody, anti mAVO3 antibody, anti pianissimo antibody, anti DKFZp686B11164 antibody, complex 2 antibody, anti PIA antibody, anti mAVO3 antibody, anti TORC2-specific protein AVO3 antibody
Reactivity
Human
Target
RICTOR
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KQPIVDTSAES
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:4000, WB: 0.1 μg/ml, IHC-P: 2-3μg/ml
Molecular Weight
192
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Mouse
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
253260
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