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RICTOR Antibody

Goat polyclonal antibody to RICTOR

Product Specifications

Product Name Alternative

Anti RICTOR antibody, anti Rictor antibody, anti KIAA1999 antibody, anti AVO3 antibody, anti MGC39830 antibody, anti likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae) antibody, anti mAVO3 antibody, anti pianissimo antibody, anti DKFZp686B11164 antibody, complex 2 antibody, anti PIA antibody, anti mAVO3 antibody, anti TORC2-specific protein AVO3 antibody

Reactivity

Human

Target

RICTOR

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KQPIVDTSAES

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 0.1 μg/ml, IHC-P: 2-3μg/ml

Molecular Weight

192

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Mouse

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

253260

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