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AP-2 gamma Antibody

Goat polyclonal antibody to TFAP2C

Product Specifications

Product Name Alternative

Anti TFAP2C antibody, anti ERF1 antibody, anti TFAP2G antibody, anti hAP-2g antibody, anti AP2-GAMMA antibody, anti transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) antibody, anti estrogen receptor factor 1 antibody, anti transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma) antibody

Reactivity

Human

Target

AP-2 gamma

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NKTLEKMEKHRK

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1 μg/ml, IHC-P: 2-3μg/ml

Molecular Weight

49.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.WB: WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

7022

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