AP-2 gamma Antibody
Goat polyclonal antibody to TFAP2C
Product Specifications
Product Name Alternative
Anti TFAP2C antibody, anti ERF1 antibody, anti TFAP2G antibody, anti hAP-2g antibody, anti AP2-GAMMA antibody, anti transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) antibody, anti estrogen receptor factor 1 antibody, anti transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma) antibody
Reactivity
Human
Target
AP-2 gamma
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NKTLEKMEKHRK
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.1 μg/ml, IHC-P: 2-3μg/ml
Molecular Weight
49.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.WB: WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
7022
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