Goat anti-CD274 / PD-L1 Antibody
Goat polyclonal antibody to CD274
Product Specifications
Product Name Alternative
Anti-CD274 antibody, anti-Programmed cell death 1 ligand 1 antibody, anti-Programmed death ligand 1 antibody, anti-B7 homolog 1 antibody, anti-B7-H1 antibody.
Reactivity
Human
Target
CD274 / PD-L1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CKKQSDTHLEET
Field of Research
Immunology & Inflammation; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.01-0.1 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
Molecular Weight
33.3; 20.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Skel; etal Muscle shows staining of the striated muscular fibres. Recommended concentration, 4-6ug/ml. Data obtained with a previous batch.WB: Approx 35kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1) . Recommended concentration: 0.3-1.0 μg/ml.
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
29126
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