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Cytochrome b reductase 1 Antibody

Goat polyclonal antibody to CYBRD1

Product Specifications

Product Name Alternative

Anti CYBRD1 antibody, anti DCYTB antibody, anti FLJ23462 antibody, anti cytochrome b reductase 1 antibody, anti duodenal cytochrome b antibody, anti FRRS3 antibody, anti ferric-chelate reductase 3 antibody

Reactivity

Human

Target

Cytochrome b reductase 1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CRNLALDEAGQRSTM

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.1 μg/ml, IHC-P: 2.5ug/ml

Molecular Weight

31.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

79901

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