PDE5A Antibody
Goat polyclonal antibody to PDE5A
Product Specifications
Product Name Alternative
Anti PDE5A antibody, anti CN5A antibody, anti PDE5 antibody, anti PDE5A1 antibody, anti CGB-PDE antibody, anti phosphodiesterase 5A, cGMP-specific antibody, anti cGMP-binding cGMP-specific 3\1,5\1-cyclic nucleotide phosphodiesterase antibody
Reactivity
Human
Target
PDE5A
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
LINGESGQAKRN
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
Molecular Weight
100; 94.8; 93.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 100kDa band observed in Human Lung lysates (calculated MW of 100kDa according to isoform 1, NP_001074.2) . In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2) . Recommended concentration: 0.3-1 μg/ml.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
8654
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