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Goat anti-AMID / PRG3 Antibody

Goat polyclonal antibody to AIFM2

Product Specifications

Product Name Alternative

Anti AMID antibody, anti PRG3 antibody, anti apoptosis-inducing factor (AIF) -like mitochondrion-associated inducer of death antibody, anti 5430437E11Rik antibody, anti apoptosis-inducing factor (AIF) -homologous mitochondrion-associated inducer of death antibody, anti AIFM2 antibody, anti apoptosis-inducing factor, mitochondrion-associated, 2 antibody, anti RP11-367H5.2 antibody, anti OTTHUMP00000019740 antibody, anti apoptosis-inducing factor (AIF) -homologous mitochondrion-associated inducer of death antibody

Reactivity

Human

Target

AMID / PRG3

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

GSQVSVESGALHC

Field of Research

Metabolism Research; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1 μg/ml, IHC-P: 4 μg/ml

Molecular Weight

40.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 48-50kDa band in Human Colon and Duodenum lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.5kDa according to NP_116186. The 48-50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

84883

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