Centromere protein F/mitosin Antibody
Goat polyclonal to Centromere protein F
Product Specifications
Product Name Alternative
Anti CENPF antibody, anti centromere protein F (350/400kD, mitosin) antibody, anti CENF antibody, anti PRO1779 antibody, anti mitosin antibody, anti AH antigen antibody, anti CENP-F kinetochore protein antibody, anti centromere protein F (400kD) antibody, anti cell-cycle-dependent 350K nuclear protein antibody, anti centromere protein F, 350/400ka (mitosin) antibody, anti RP11-262H5.1 antibody, anti hcp-1 antibody, anti centromere protein F antibody, anti mitosin antibody
Reactivity
Human
Target
Centromere protein F / mitosin
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
GLESKGSENCKVQ
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, IHC-P: 3.75 μg/ml
Molecular Weight
358
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1063
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