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DKK1 Antibody

Goat polyclonal antibody to DKK1

Product Specifications

Product Name Alternative

Anti DKK1 antibody, anti SK antibody, anti DKK-1 antibody, anti dickkopf homolog 1 (Xenopus laevis) antibody, anti dickkopf-1 like antibody, anti dickkopf (Xenopus laevis) homolog 1 antibody, anti dickkopf homolog 1 antibody, anti dickkopf related protein-1 antibody

Reactivity

Human

Target

DKK1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CDHHQASNSSRLHT

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

10 μg/ml

Dilution

ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

28.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa band observed in human spleen and bone marrow lysates after 1 μg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463) . However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/ml.

Prediction Reactivity

Bovine, Mouse

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

22943

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