DKK1 Antibody
Goat polyclonal antibody to DKK1
Product Specifications
Product Name Alternative
Anti DKK1 antibody, anti SK antibody, anti DKK-1 antibody, anti dickkopf homolog 1 (Xenopus laevis) antibody, anti dickkopf-1 like antibody, anti dickkopf (Xenopus laevis) homolog 1 antibody, anti dickkopf homolog 1 antibody, anti dickkopf related protein-1 antibody
Reactivity
Human
Target
DKK1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CDHHQASNSSRLHT
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
10 μg/ml
Dilution
ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
Molecular Weight
28.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa band observed in human spleen and bone marrow lysates after 1 μg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463) . However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/ml.
Prediction Reactivity
Bovine, Mouse
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
22943
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