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PHLDA3/TIH1 Antibody

Goat polyclonal antibody to PHLDA3

Product Specifications

Product Name Alternative

Anti PHLDA3 antibody, anti TIH1 antibody, anti pleckstrin homology-like domain, family A, member 3 antibody, anti pleckstrin homology-like domain, family A, member 2 antibody, anti OTTHUMP00000033920 antibody

Reactivity

Human

Target

PHLDA3 / TIH1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RARQSLGTGTLVS

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.2-1 μg/ml

Molecular Weight

13.9

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 70kDa band in Human Placenta lysate after 1 μg/ml antibody staining. This band was successfully blocked by the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13.9kDa according to NP_036528. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

23612

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