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Pirin Antibody

Goat polyclonal antibody to PIR

Product Specifications

Product Name Alternative

Anti pirin antibody, anti PIR antibody, anti pirin (iron-binding nuclear protein) antibody

Reactivity

Human

Target

Pirin

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

GSSKKVTLSVLSREC

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.3 - 1.0 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

32.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 33kDa band observed in human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1) . In transfected HEK293 transiently expressing PIR a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1.0 μg/ml.

Prediction Reactivity

Mouse

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

8544

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