Pirin Antibody
Goat polyclonal antibody to PIR
Product Specifications
Product Name Alternative
Anti pirin antibody, anti PIR antibody, anti pirin (iron-binding nuclear protein) antibody
Reactivity
Human
Target
Pirin
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
GSSKKVTLSVLSREC
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.3 - 1.0 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
32.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 33kDa band observed in human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1) . In transfected HEK293 transiently expressing PIR a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1.0 μg/ml.
Prediction Reactivity
Mouse
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
8544
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