MitoPT TMRE Mitochondrial Depolarization Assay Kit

1. Prepare samples. 2. Create controls with CCCP. 3. Dilute 10X Assay Buffer 1:10 with diH2O. 4. Reconstitute MitoPT TMRM with DMSO. 5. Dilute MitoPT TMRM with 1X Assay Buffer. 6. Add MitoPT TMRM to each sample. 7. Incubate 15-30 minutes. 8. Wash cells: add 1X Assay Buffer and spin cells. 9. Remove supernatant and resuspend cells for analysis. 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT TMRM excites at 548 nm and emits at 573 nm (orange) .