MitoPT TMRM Mitochondrial Depolarization Assay Kit
TMRM accumulates and fluoresces orange in polarized mitochondria. Fluorescence drops dramatically on depolarization in apoptotic or metabolically stressed cells. Analyze using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
Product Specifications
Sample Type
Cell culture
Storage Conditions
-20°C
Notes
For research use only.
Applications Notes
1. Prepare samples and controls. 2. Dilute 10X Injection Buffer 1:10 with 45 mL diH20. 3. Reconstitute NIR-FLIVO 690 Tracer with 50 μL DMSO. 4. Dilute NIR-FLIVO 1:10 with 450 μL 1X Injection Buffer. 5. Inject 50 μL intravenously. 6. Let NIR-FLIVO circulate for 4 hours. 7. Image the animal. NIR-FLIVO 690 Tracer excites at 690 nm and emits at 709 nm. 8. If not viewing directly, excise tissue and process samples for further analysis. 9. Optional: label excised samples with additional stains, such as Hoechst 33342, or an antibody. 10. If desired, fix excised samples. 11. Analyze excised samples with a fluorescence microscope or flow cytometer.
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