FLICA 660 Caspase 3/7 Assay Kit
This in vitro assay employs the far-red fluorescent inhibitor probe 660-DEVD-FMK to label active caspase 3 and caspase 7 enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.
Product Specifications
Field of Research
Cell Biology
Sample Type
Cell culture
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare Assay buffer; dilute Assay Buffer Concentrate 1:5 in diH2O. 2. Prepare samples; dilute > 1:10 in Assay Buffer. 3. Prepare standard curve and zero (blank) standard. 4. Add 50 μL blanks, standards, and samples to plate. 5. Add 25 μL Fluorescent H2O2 Detection Substrate to plate. 6. Prepare HRP; dilute Horseradish Peroxidase Concentrate 1:100 in Assay Buffer. 7. Add 25 μL HRP Preparation to plate. 8. Incubate 15 minutes at room temperature. 9. Read fluorescence with excitation at 570 nm and emission at 590 nm.
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