Antigen-Down ELISA Development Kit
This kit provides seven specially formulated ELISA solutions and a template for the initial development and optimization of antigen-down immunoassays by both novice and experienced users. The kit contains enough reagent for ten 96-well ELISA plates.
Product Specifications
Field of Research
Immunology & Inflammation
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare samples. 2. Create controls with CCCP. 3. Dilute 10X Assay Buffer 1:10 with diH2O. 4. Reconstitute MitoPT TMRE with DMSO. 5. Dilute MitoPT TMRE with 1X Assay Buffer. 6. Add MitoPT TMRE to each sample. 7. Incubate 15-30 minutes. 8. Wash cells: add 1X Assay Buffer and spin cells. 9. Remove supernatant and resuspend cells for analysis. 10. Anazlyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT TMRE excites at 549 nm and emits at 574 nm (orange) .
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