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FAM-FLICA Poly Caspase Assay Kit

This FLICA (Fluorescent Labeled Inhibitors of CAspases) kit is designed to detect active caspase enzymes. This in vitro assay employs the fluorescent inhibitor probe FAM-VAD-FMK to label active caspase enzymes in living cells. Samples can be analyzed using fluorescence microscopy, a fluorescence plate reader, or by flow cytometry. Our Green Fluorescent Poly (active) Caspase FAM FLICA Inhibitor Reagent (FAM-VAD-FMK) can be used as a general reagent to detect apoptosis as it is recognized by many types of activated caspases. The probe measures the intracellular process of apoptosis instead of a side-effect, such as the turnover of phosphatidyl serine, and eliminates the incidence of false positives that often plagues methods like Annexin V and TUNEL staining.

Product Specifications

Field of Research

Cell Biology

Sample Type

Cell culture

Storage Conditions

2-8°C

Notes

For research use only.

Applications Notes

1. Prepare samples and controls. 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20. 3. Reconstitute FLICA with 50 μL DMSO. 4. Dilute FLICA 1:5 by adding 200 μL PBS. 5. Add diluted FLICA to each sample at 1:30 (e.g. add 10 μL to 290 μL of cultured cells) . 6. Incubate approximately 1 hour. 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells. 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody. 9. If desired, fix cells. 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm. If working with adherent cells, please see the manual for additional protocols.

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