SR FLICA Caspase 3 and 7 Assay Kit

1. Prepare samples and controls. 2. Reconstitute Magic Red with 100 or 400 μL DMSO, 3. Dilute Magic Red 1:5 with diH2O, 4. Add diluted Magic Red at 1:15-1:30 to suspension cells, 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes of addition of Magic Red, 7. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody. 8. Analyze with a fluorescent microscope, fluorescent plate reader, or flow cytometer. Magic Red has a maximum excitation at 592 nm and emission at 628 nm. Good fluorescence images can be obtained at 510-560 nm excitation and >610 nm emission. If working with adherent cells, please see the manual for additional protocols.