Magic Red Cathepsin B Assay Kit

1. Prepare samples and controls. 2. Reconstitute Magic Red by adding 50 μL DMSO. 3. Dilute Magic Red 1:10 by adding 450 μL diH2O. 4. Add 20 μL Magic Red to each sample (~ 500 μL aliquot of cultured cells) . 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes. 7. If desired, label with additional stains, such as Hoechst, DAPI, Acridine orange, or an antibody. 8. If desired, fix or embed cells. 9. Analyze with a fluorescence microscope or fluorescence plate reader. Magic Red has an optimal excitation at 592 nm and emission at 628 nm. If working with adherent cells, please see the manual for additional protocols.