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Goat anti-RASIP1 / RAIN Antibody

Goat polyclonal to RASIP1

Product Specifications

Product Name Alternative

Anti RASIP1 antibody, anti Ras-interacting protein 1 antibody, anti RAIN antibody, anti FLJ20401 antibody, anti Ras interacting protein 1 antibody

Reactivity

Human

Target

RASIP1 / RAIN

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CANYRHGPPVATSP

Field of Research

Cardiovascular Research; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.3 ug/ml, IHC-P: 3.75 μg/ml

Molecular Weight

103.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size of 110kDa according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

54922

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