Recombinant Human B-lymphocyte antigen CD20 (MS4A1) -Detergent (Active)

Product Specifications

Product Name Alternative

(B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD20)

Abbreviation

Recombinant Human MS4A1 protein-Detergent (Active)

Gene Name

MS4A1

UniProt

P11836

Expression Region

1-297aa

Organism

Homo sapiens (Human)

Target Sequence

MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP

Tag

C-terminal 10xHis-tagged

Type

MP-Detergent Transmembrane Protein & Active Protein & In Stock Protein

Source

Mammalian cell

Field of Research

Cancer

Relevance

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111) . Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602) .

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Yes

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007A1HU), the EC50 is 4.034-6.968 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Human CD20 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC50 is 8.137-9.704 ng/mL.

Form

Liquid

Buffer

50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, pH7.5

Reconstitution

Molecular Weight

34.4 kDa

References & Citations

"CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins." Polyak M.J., Li H., Shariat N., Deans J.P. J. Biol. Chem. 283:18545-18552 (2008)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.